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A Conversation with Duke’s Dr. Xiaoyin Sara Jiang
To determine the efficacy of a plant-protective insecticidal agent it must be tested on its targeted pest as well as in combination with the plant it is expected to protect. To achieve this, experiments are performed in vitro to screen through thousands of potentially active compounds or microbial strains. This is done by exposing the animal, in this case caterpillars, to an environment pre-treated with the insecticidal agent(s). Two common assays are used to assess the efficacy: the Diet Assay and the Leaf Disk assay. Both of these assays are performed in multi well plates so as to test multiple agents at different concentrations and with repetition to gain statistical confidence in the results.
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