RAMONA
We built Ramona to advance human health through live imaging that delivers unrivaled precision and scale

Our goals


Here, we present reflective Fourier light field computed tomography (ReFLeCT), a high-speed volumetric fluorescence computational imaging technique. ReFLeCT synchronously captures entire tomograms of multiple unrestrained, unanesthetized model organisms across multi-millimeter 3D FOVs at 120 volumes per second. In particular, we applied ReFLeCT to reconstruct 4D videos of fluorescently labeled zebrafish and Drosophila larvae, enabling us to study their heartbeat, fin and tail motion, gaze, jaw motion, and muscle contractions with nearly isotropic 3D resolution while they are freely moving.
Patient-derived organoids (PDOs) are a valuable tool for investigations of intra-tumor and inter-site heterogeneity and patient-specific drug responsiveness. In this work, we employ the MCAM (Multi-Camera Array Microscope) Vireo™ system to rapidly acquire brightfield images of PDOs derived from gastrointestinal surgical resection samples in under 2 minutes per 24 well plate and a new machine learning model to automatically analyze this data.
We present a multi-camera array for capturing dynamic high-resolution videos of the human face. Compared to traditional single-camera configurations, our array of 54 individual cameras allows stitching of high-resolution composite video frames (709 megapixels total). In our novel multi-focus strategy, each camera in the array focuses on a unique object plane to resolve non-planar surfaces at a higher resolution than a standard single-lens camera design. Compared to a single-focus configuration, this is almost a 10-fold increase in effective DOF. We demonstrate how our multi-focus camera array can capture dynamic facial expressions at microscopic resolution with relevance in several biomedical applications.
.avif)